A larval survey of dengue vectors was conducted from July to November 1996 and from May to November 1997 in Chiangmai Province, Thailand. Three villages in urban, transition, and rural areas were selected for the survey to clarify the spatial distribution of Ae. aegypti and Ae. albopictus along an urban–rural ecological gradient. The average number of Ae. aegypti larvae in larvitraps was higher in the urban area than in the rural area, as we expected, whereas the opposite was found for Ae. albopictus, rural area > urban area. A house survey of larvae-inhabiting containers showed significant differences in the number and composition of these containers among the study areas. Significant differences also were found in the average distance between houses, average tree height, and average percentage of vegetation cover for each house. The seasonal pattern of rainfall recorded in each study area did not show great differences among the study areas. The response of Ae. aegypti and Ae. albopictus to the urban–rural gradient is discussed in relation to the possibility of applying geographic information system techniques to plan the control strategy and surveillance of dengue vectors.